Results
| PMID | 7962280 |
| Gene Name | EGFR |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 30 |
| Population details | 30 (6 eutopic endometrium, 10 cyst walls of endometriomas, 9 endometriotic implants, 5 pelvic adhesions) |
| Sex | Female |
| Associated genes | EGF-R |
| Other associated phenotypes |
Endometriosis |
|
J Clin Endocrinol Metab. 1994 Oct;79(4):1097-101. Huang, J C| Yeh, J Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, Massachusetts 02115. The molecular mechanisms by which endometriosis persists in locations outside the uterus are unclear. Recently, the epidermal growth factor receptor (EGF-R) has been postulated to have a role in the disease process of endometriosis. To explore this, we determined the levels of EGF-R protein and messenger ribonucleic acid (mRNA) expression in endometriotic tissues and compared the levels to that of eutopic endometrium. Using rabbit anti-EGF-R antibody, we found more intense immunohistochemical staining for EGF-R in glandular cells than in stromal cells of both endometriomas and endometriotic implants. No difference in staining intensity was noted between endometriotic tissues and eutopic endometrium. A ribonuclease protection assay was used to determine mRNA levels for EGF-R. PhosphoImager analysis revealed the following levels of mRNA for EGF-R; eutopic endometrium, 1.00 +/- 0.27 (arbitrary units; mean +/- SEM; n = 6 patients); cyst walls of endometriomas, 0.21 +/- 0.12 (n = 10 patients); endometriotic implants, 0.29 +/- 0.13 (n = 9 patients); and pelvic adhesions, 0.03 +/- 0.03 (n = 5 patients). Endometriotic tissues had significantly less mRNA for EGF-R than eutopic endometrium (P < 0.05, by Newman-Keuls test). Our findings support the hypothesis that EGF-R may be associated with the disease process of endometriosis. Mesh Terms: Endometriosis/*genetics/metabolism| Female| *Gene Expression| Humans| Immunohistochemistry| Nucleic Acid Hybridization| RNA, Messenger/metabolism| Receptor, Epidermal Growth Factor/*genetics/metabolism| Ribonucleases|DA 1994/10/01 00:01 |
|